… PCR-ELISA being a laborious and expensive method was modified and simplified by using
… of citrus tristeza virus isolates from cyprus on the basis of the coat protein gene. Journal of …

… of the most devastating citrus viruses ie citrus tristeza virus. … IC-RT-PCR was used to
amplify the coat protein gene [112]. … PCR-ELISA being a laborious and expensive method was …

… Polar CTV virions consist mainly of two capsid proteins, CP and CPm (minor capsid
protein)… Symptoms were registered periodically after new growth flushes: VC and LC in ‘Mexican’ …

… , major and minor coat proteins (CP, 25-kDa, and CPm-kDa, … ) affect the growth and
development of certain citrus cultivars such … This type of grouping or classification leads to …

… Fonseca, “Development of an asymmetric PCR-ELISA typing method for citrus tristeza
virus based on the coat protein gene,” Journal of Virological Methods, vol. 155, no. 2, pp. …

… erefore, our aim was to develop an approach that could be applied to individual samples, …
asymmetric PCR-ELISA typing method for citrus tristeza virus based on the coat protein gene…

… Development of an asymmetric PCR-ELISA typing method for Citrus tristeza virus based
on the coat protein gene… Journal of Virological Methods, 155, 97–108. …

… within 12–80% depending on type of crop ranging from maize, … to coat protein region of
virus) in presence of infecting virus … methods for this analysis have been reportedly developed …

… [48] who developed an asymmetric PCR-ELISA typing (APET) assay for the coat protein
gene of isolates of citrus tristeza virus. The integration of asymmetric PCR with aptamers allows …

… ringspot virus, citrus tristeza virus, cacao mild mosaic virus, … Subsequent analyses of coat
protein gene sequences of PRSV … traditional methods of developing transgenic virus resistance …